Anti-UCP3 antibody
| Name | Anti-UCP3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10985 |
| Prices | $382.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to amino acids 254-267 of Human UCP3 with N-terminal lysine added |
| Description | Rabbit Polyclonal |
| Gene | UCP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab10985 (2µg/ml) staining UCP3 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of myocytes.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
Transcriptional analysis of brown adipose tissue in leptin-deficient mice lacking - Transcriptional analysis of brown adipose tissue in leptin-deficient mice lacking
Becerril S, Rodriguez A, Catalan V, Sainz N, Ramirez B, Gomez-Ambrosi J, Fruhbeck G. Physiol Genomics. 2012 Jul 3;44(13):678-88. doi:
Deletion of inducible nitric-oxide synthase in leptin-deficient mice improves - Deletion of inducible nitric-oxide synthase in leptin-deficient mice improves
Becerril S, Rodriguez A, Catalan V, Sainz N, Ramirez B, Collantes M, Penuelas I, Gomez-Ambrosi J, Fruhbeck G. PLoS One. 2010 Jun 4;5(6):e10962.
Effect of exercise and calorie restriction on biomarkers of aging in mice. - Effect of exercise and calorie restriction on biomarkers of aging in mice.
Huffman DM, Moellering DR, Grizzle WE, Stockard CR, Johnson MS, Nagy TR. Am J Physiol Regul Integr Comp Physiol. 2008 May;294(5):R1618-27. doi: