All lanes : Anti-UCP3 antibody (ab125830) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) - (Negative Control)Lane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)- (Negative Control)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.