All lanes : Anti-UFD1L antibody [EPR12847] - N-terminal (ab181080) at 1/2000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLysates/proteins at 1/1000 dilution per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling UFD1L with ab181080 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight 488) at 1/200 dilution. Conterstained with Dapi (blue).
Flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling UFD1L with ab181080 at 1/30 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit IgG