Flow cytometric analysis of NCI-H460 cells labeling UGT2B15 with ab170750 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of NCI-H460 cells labeling UGT2B15 with ab170750 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling UGT2B15 with ab170750 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
All lanes : Anti-UGT2B15 antibody (ab170750) at 1/100 dilutionLane 1 : NCI-H460 cell line lysateLane 2 : HepG2 cell line lysateLane 3 : ZR-75-1 cell line lysateLysates/proteins at 35 µg per lane.