Anti-UHRF1 antibody
| Name | Anti-UHRF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57083 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: WNEVLASLKD RPASGSPFQL FLSKVEETFQ CICCQELVFR PITTVCQHNV CKDCLDRSFR AQVFSCPACR YDLGRSYAMQ VNQPLQTVLN QLFPGYGNGR , corresponding to amino acids 694-794 of Human UHRF1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | UHRF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
UHRF1 antibody (ab57083) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.
ICC/IF image of ab57083 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57083, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing MCF7 cells stained with ab57083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/17960_UHRF1-Primary-antibodies-ab57083-2.jpg)
Overlay histogram showing MCF7 cells stained with ab57083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
UHRF1 promotes cell growth and metastasis through repression of p16(ink(4)a) in - UHRF1 promotes cell growth and metastasis through repression of p16(ink(4)a) in
Wang F, Yang YZ, Shi CZ, Zhang P, Moyer MP, Zhang HZ, Zou Y, Qin HL. Ann Surg Oncol. 2012 Aug;19(8):2753-62.
Association of UHRF1 with methylated H3K9 directs the maintenance of DNA - Association of UHRF1 with methylated H3K9 directs the maintenance of DNA
Rothbart SB, Krajewski K, Nady N, Tempel W, Xue S, Badeaux AI, Barsyte-Lovejoy D, Martinez JY, Bedford MT, Fuchs SM, Arrowsmith CH, Strahl BD. Nat Struct Mol Biol. 2012 Nov;19(11):1155-60.
Nucleosome-interacting proteins regulated by DNA and histone methylation. - Nucleosome-interacting proteins regulated by DNA and histone methylation.
Bartke T, Vermeulen M, Xhemalce B, Robson SC, Mann M, Kouzarides T. Cell. 2010 Oct 29;143(3):470-84.