All lanes : Anti-ULBP1 antibody [EPR7532(2)] (ab176566) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Flow cytometry analysis of permeabilized K562 cells labeling ULBP1 (red) with ab176566 at a 1/100 dilution, or negative control rabbit IgG (green)
All lanes : Anti-ULBP1 antibody [EPR7532(2)] (ab176566) at 1/1000 dilutionLane 1 : Lysate from CHO cells mock-transfectedLane 2 : Lysate from CHO cells transfected with ULBP1Lysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique