Anti-ULBP4 antibody [EPR17443]

• Supplier: Abcam
• Catalog: ab200662
• Prices: $99.00, $347.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17443
• Applications: WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human ULBP4 aa 1-250
• Description: Rabbit Monoclonal
• Gene: RAET1E
• Conjugate: Unconjugated

Product images

All lanes : Anti-ULBP4 antibody [EPR17443] (ab200662) at 1/5000 dilutionLane 1 : HepG2 (Human liver hepatocellular carcinoma) cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-ULBP4 antibody [EPR17443] (ab200662) at 1/1000 dilution + Human fetal brain lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-ULBP4 antibody [EPR17443] (ab200662) at 1/1000 dilutionLane 1 : C6 (Rat glial tumor cells) cell lysateLane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysateLane 3 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysateLane 4 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

ULBP4 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200662 at 1/100 dilution.Lane 1: HeLa whole cell lysate 10 µg (Input).Lane 2: ab200662 IP in HeLa whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200662 in HeLa whole cell lysate.Western blot was performed using ab200662 at 1/2000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Blocking and dilution buffer and concentration: 5% NFDM/TBST.