Anti-UNC5D antibody
| Name | Anti-UNC5D antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58141 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB ICC/IF ICC/IF FC IHC |
| Species Reactivities | Mouse, Chicken, Human |
| Antigen | Recombinant fragment: TDNGEALPES IPSAPGTLPH FIEEPDDAYI IKSNPIALRC KARPAMQIFF KCNGEWVHQN EHVSEETLDE SSGLKVREVF INVTRQQVED FHGPEDYWCQ , corresponding to amino acids 31-131 of Human UNC5D Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | UNC5D |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing SH-SY5Y cells stained with ab58141 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58141, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18104_ab58141-3-ab58141FC.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab58141 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58141, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Western blot against tagged recombinant protein immunogen using ab58141 UNC5D antibody at 1ug/ml.
ab58141 staining UNC5D in chicken whole mount brain by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 10% serum for 48 hours. Samples were then incubated with primary antibody at 1/500 for 48 hours. The secondary antibody used was a goat anti-mouse monoclonal conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.See Abreview
Product References
Tbr2 is required to generate a neural circuit mediating the pupillary light - Tbr2 is required to generate a neural circuit mediating the pupillary light
Sweeney NT, Tierney H, Feldheim DA. J Neurosci. 2014 Apr 16;34(16):5447-53.
A network of heterochronic genes including Imp1 regulates temporal changes in - A network of heterochronic genes including Imp1 regulates temporal changes in
Nishino J, Kim S, Zhu Y, Zhu H, Morrison SJ. Elife. 2013 Nov 5;2:e00924.