Anti-UNG antibody
| Name | Anti-UNG antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23926 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human, Mouse, Rat, Chicken, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UNG |
| Description | Rabbit Polyclonal |
| Gene | UNG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-UNG antibody (ab23926) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : HeLa (Human epithelial carcinoma cell line) nuclear lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
ICC/IF image of ab23926stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23926, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Image courtesy of Human Protein Atlasab23926 staining UNG in human heart muscle. Paraffin embedded human heart muscle tissue was incubated with ab23926 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23926 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Product References
Uracil DNA glycosylase counteracts APOBEC3G-induced hypermutation of hepatitis B - Uracil DNA glycosylase counteracts APOBEC3G-induced hypermutation of hepatitis B
Kitamura K, Wang Z, Chowdhury S, Simadu M, Koura M, Muramatsu M. PLoS Pathog. 2013;9(5):e1003361.
Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: - Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity:
Bulgar AD, Weeks LD, Miao Y, Yang S, Xu Y, Guo C, Markowitz S, Oleinick N, Gerson SL, Liu L. Cell Death Dis. 2012 Jan 12;3:e252.
Recruitment of the nuclear form of uracil DNA glycosylase into virus particles - Recruitment of the nuclear form of uracil DNA glycosylase into virus particles
Guenzel CA, Herate C, Le Rouzic E, Maidou-Peindara P, Sadler HA, Rouyez MC, Mansky LM, Benichou S. J Virol. 2012 Mar;86(5):2533-44.
Human immunodeficiency virus type 1 Vpr modulates cellular expression of UNG2 via - Human immunodeficiency virus type 1 Vpr modulates cellular expression of UNG2 via
Langevin C, Maidou-Peindara P, Aas PA, Jacquot G, Otterlei M, Slupphaug G, Benichou S. J Virol. 2009 Oct;83(19):10256-63.
Ugene, a newly identified protein that is commonly overexpressed in cancer and - Ugene, a newly identified protein that is commonly overexpressed in cancer and
Guo C, Zhang X, Fink SP, Platzer P, Wilson K, Willson JK, Wang Z, Markowitz SD. Cancer Res. 2008 Aug 1;68(15):6118-26.
Cell cycle regulation as a mechanism for functional separation of the apparently - Cell cycle regulation as a mechanism for functional separation of the apparently
Hardeland U, Kunz C, Focke F, Szadkowski M, Schar P. Nucleic Acids Res. 2007;35(11):3859-67. Epub 2007 May 25.