Lane 1 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 0.5 µg/mlLane 2 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 1 µg/mlLane 3 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 2 µg/mlLane 1 : C2C12 cell lysate Lane 2 : C2C12 cell lysate Lane 3 : C2C12 cell lysate Lysates/proteins at 15 µg per lane.
Immunohistochemical analysis of UNG expression in human heart tissue using 2 µg/ml ab62520.
ICC/IF image of ab62520 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62520, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of UNG1 in Human Heart cells using ab62520 at 20 ug/ml.