Anti-Unrip antibody - C-terminal (ab135536) at 1/50 dilution + HeLa cell line lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labelling Unrip with ab135536 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labelling Unrip with ab135536 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-Unrip antibody - C-terminal (ab135536) at 1/50 dilutionLane 1 : 293 cell lysate, non-transfectedLane 2 : 293 cell lysate, transiently transfected with the Unrip geneLysates/proteins at 2 µg per lane.