Anti-UQCRQ antibody [1H9DE5DG5BC8]
| Name | Anti-UQCRQ antibody [1H9DE5DG5BC8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab110255 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 1H9DE5DG5BC8 |
| Applications | FC WB |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Bovine Complex III subunit 8 (UQCRQ) |
| Description | Mouse Monoclonal |
| Gene | UQCRQ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HepG2 cells stained with ab110255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110255, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18323_ab110255-2-ab110255FC.jpg)
Overlay histogram showing HepG2 cells stained with ab110255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110255, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-UQCRQ antibody [1H9DE5DG5BC8] (ab110255) at 1 µg/mlLane 1 : Human mitochondrial lysate at 10 µgLane 2 : Cow mitochondrial lysate at 1 µgLane 3 : Rat mitochondrial lysate at 10 µgLane 4 : Mouse mitochondrial lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18324_testtesttest-Primary-antibodies-ab110255-1.jpg)
All lanes : Anti-UQCRQ antibody [1H9DE5DG5BC8] (ab110255) at 1 µg/mlLane 1 : Human mitochondrial lysate at 10 µgLane 2 : Cow mitochondrial lysate at 1 µgLane 3 : Rat mitochondrial lysate at 10 µgLane 4 : Mouse mitochondrial lysate at 10 µg
Product References
How do human cells react to the absence of mitochondrial DNA? - How do human cells react to the absence of mitochondrial DNA?
Mineri R, Pavelka N, Fernandez-Vizarra E, Ricciardi-Castagnoli P, Zeviani M, Tiranti V. PLoS One. 2009 May 28;4(5):e5713.
Impaired complex III assembly associated with BCS1L gene mutations in isolated - Impaired complex III assembly associated with BCS1L gene mutations in isolated
Fernandez-Vizarra E, Bugiani M, Goffrini P, Carrara F, Farina L, Procopio E, Donati A, Uziel G, Ferrero I, Zeviani M. Hum Mol Genet. 2007 May 15;16(10):1241-52. Epub 2007 Apr 2.
Strategies to reduce late-stage drug attrition due to mitochondrial toxicity. - Strategies to reduce late-stage drug attrition due to mitochondrial toxicity.
Dykens JA, Marroquin LD, Will Y. Expert Rev Mol Diagn. 2007 Mar;7(2):161-75.