All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/100 dilution (unpurified)Lane 1 : LnCap cell lysateLane 2 : PC-3 cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/1300 dilution (purified)Lane 1 : LnCaP cell lysateLane 2 : PC-3 cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/1000 dilution (unpurified)Lane 1 : PC-3 cell lysateLane 2 : LnCaP cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Urokinase with purified ab133563 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD