![USP14 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab137432.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa; USP14](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18568_ab137432-215252-IPVI006ab13743216m.jpg)
USP14 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab137432.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa; USP14
Anti-USP14 antibody (ab137432) at 1/1000 dilution + Mouse brain lysate at 50 µg
All lanes : Anti-USP14 antibody (ab137432) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : Jurkat whole cell lysateLysates/proteins at 30 µg per lane.
Anti-USP14 antibody (ab137432) at 1/1000 dilution + Rat brain cell lyaste at 50 µg
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling USP14 with ab137432 at 1/500 dilution.