USP14

Gene Symbol USP14
Entrez Gene 9097
Alt Symbol TGT
Species Human
Gene Type protein-coding
Description ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Other Description deubiquitinating enzyme 14|tRNA-guanine transglycosylase, 60-kD subunit|ubiquitin carboxyl-terminal hydrolase 14|ubiquitin specific protease 14 (tRNA-guanine transglycosylase)|ubiquitin thioesterase 14|ubiquitin thiolesterase 14|ubiquitin-specific processing protease 14|ubiquitin-specific-processing protease 14
Swissprots J3QRZ5 P54578 B7Z4N8 Q53XY5
Accessions EAX01735 EAX01736 EAX01737 EAX01738 P54578 AK095516 BAG53076 AK098497 AK297605 BAH12624 AK315438 BAG37826 BC003556 AAH03556 BG717018 BT007183 AAP35847 BU161860 CA438203 CR976282 DQ890594 ABM92211 DQ893762 ABM84688 U30888 AAB60365 NM_001037334 NP_001032411 NM_005151 NP_005142
Function Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). {ECO:0000269|PubMed:18162577, ECO:0000269|PubMed:19106094, ECO:0000269|PubMed:19135427}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:19106094}. Cell membrane {ECO:0000269|PubMed:19106094}; Peripheral membrane protein {ECO:0000269|PubMed:19106094}.

USP14 (D8Q6S) Rabbit mAb - 11931 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8Q6S
Western blot analysis of extracts from various cell lines using USP14 (D8Q6S) Rabbit mAb.

USP14 Antibody - 8159 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using USP14 Antibody.

USP14 (F-4) - sc-398009 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP14 (F-4): sc-398009. Western blot analysis of USP14 expression in HeLa (A) and MOLT-4 (B) whole cell lysates.

USP14 (C-16) - sc-69074 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
USP14 (C-16): sc-69074. Western blot analysis of USP14 expression in Jurkat (A), MOLT-4 (B) and RAW 264.7 (C) whole cell lysates.

USP14 (E-17) - sc-69075 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
USP14 (E-17): sc-69075. Western blot analysis of USP14 expression in non-transfected: sc-117752 (A) and human USP14 transfected: sc-174419 (B) 293T whole cell lysates.

USP14 (H-300) - sc-292678 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

USP14 (6E6) - sc-100630 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
USP14 (6E6): sc-100630. Western blot analysis of USP14 expression in HeLa whole cell lysate.

USP14 (A-1) - sc-393872 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP14 (A-1): sc-393872. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

Anti-USP14 antibody - ab137432 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
USP14 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab137432.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa; USP14

Anti-USP14 antibody - ab71165 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP14 with ab71165 at 1/1000 (1µg/ml). Detection: DAB.

Anti-USP14 antibody - ab56427 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
USP14 antibody (ab56427) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-USP14 antibody - ab56210 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-USP14 antibody [EPR15943] - C-terminal - ab192618 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15943
Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling USP14 with ab192618 at 1/70 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody.  Counterstain: DAPI.

Anti-USP14 Antibody (C-Terminus) - LS-C47055 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (aa355-383, AP) - LS-C273163 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (aa355-383, Biotin) - LS-C273164 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (aa355-383, APC) - LS-C273165 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (aa355-383, FITC) - LS-C273166 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (aa355-383, Azide-free, HRP) - LS-C273167 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (aa355-383, PE) - LS-C273168 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (N-Terminus, AP) - LS-C284172 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (N-Terminus, Biotin) - LS-C284173 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (N-Terminus, APC) - LS-C284171 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (N-Terminus, FITC) - LS-C284174 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP14 Antibody (N-Terminus, Azide-free, HRP) - LS-C284175 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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