Anti-USP14 antibody
| Name | Anti-USP14 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56427 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | FC WB |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: PQKEVKYEPF SFADDIGSNN CGYYDLQAVL THQGRSSSSG HYVSWVKRKQ DEWIKFDDDK VSIVTPEDIL RLSGGGDWHI AYVLLYGPRR VEIMEEESEQ , corresponding to amino acids 395-495 of Human USP14 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | USP14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
USP14 antibody (ab56427) at 1ug/lane + HeLa cell lysate at 25ug/lane.
![Overlay histogram showing HeLa cells stained with ab56427 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56427, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18580_USP14-Primary-antibodies-ab56427-1.jpg)
Overlay histogram showing HeLa cells stained with ab56427 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56427, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.