Anti-USP14 antibody [EPR15943] - C-terminal

• Supplier: Abcam
• Catalog: ab192618
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15943
• Applications: WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human USP14 aa 350 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: USP14
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling USP14 with ab192618 at 1/70 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody.  Counterstain: DAPI.

All lanes : Anti-USP14 antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : HCT-116 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-USP14 antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling USP14 with ab192618 at 1/70 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.