Anti-USP15 antibody
| Name | Anti-USP15 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56900 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-236 of Human USP15 |
| Description | Mouse Monoclonal |
| Gene | USP15 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab56900 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56900, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18589_ab56900-3-ab56900FC.jpg)
Overlay histogram showing HeLa cells stained with ab56900 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56900, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
USP15 antibody (ab56900) used in immunofluorescence at 10ug/ml on HeLa cells.
USP15 antibody (ab56900) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
Product References
The ubiquitin-specific protease USP15 promotes RIG-I-mediated antiviral signaling - The ubiquitin-specific protease USP15 promotes RIG-I-mediated antiviral signaling
Pauli EK, Chan YK, Davis ME, Gableske S, Wang MK, Feister KF, Gack MU. Sci Signal. 2014 Jan 7;7(307):ra3.
The U4/U6 recycling factor SART3 has histone chaperone activity and associates - The U4/U6 recycling factor SART3 has histone chaperone activity and associates
Long L, Thelen JP, Furgason M, Haj-Yahya M, Brik A, Cheng D, Peng J, Yao T. J Biol Chem. 2014 Mar 28;289(13):8916-30.