Flow cytometric analysis of Jurkat cells (left histogram) labeling USP19 with ab189518 at 1/250 dilution compared to negative control cells (right histogram). Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.
Anti-USP19 antibody [EPR14816] (ab189518) at 1/5000 dilution + Human fetal brain lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-USP19 antibody [EPR14816] (ab189518) at 1/10000 dilutionLane 1 : Jurkat lysateLane 2 : 293T lysateLane 3 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique