All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : A673 cell lysateLane 3 : BxPC-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/1000 dilutionLane 1 : Raw264.7 cell lysateLane 2 : PC-12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometry analysis of USP25 expression in 2% paraformaldehyde-fixed A673 cells using ab187156 at 1/60 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody.