All lanes : Anti-USP28 antibody (ab188240) at 1/2000 dilutionLane 1 : Lysate from U2OS cells transfected with a control siLane 2 : Lysate from U2OS cells transfected with a USP28 siLane 3 : NIH 3T3 cell lysateLane 4 : INS-1 cell lysateLysates/proteins at 40 µg per lane.
Western blot analysis (using ab188240) of immunoprecipitates from U2OS cell extractsLane 1: InputLane 2: immunoprecipitate with control serum+protein A sepharose.Lane 3: immunoprecipitate with ab188240 + protein A sepharose.
Immunofluorescent analysis of 3% PFA-fixed U2OS cells transfected with either a control si (left) or USP28 si (right) labeling USP28 with ab188240 at 1/2000 dilution.