Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP28 with ab70893 at 1/1000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-USP28 antibody (ab70893) at 0.04 µg/mlLane 1 : Whole cell lysate from 293T cells at 50 µgLane 2 : Whole cell lysate from 293T cells at 15 µgLane 3 : Whole cell lysate from 293T cells at 5 µgdeveloped using the ECL technique
Detection of Human USP28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70893 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Note: bands between 55 and 71 kDa represent the IgG chains.