All lanes : Anti-USP3 antibody (ab101473) at 1 µg/mlLane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 2 : : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab101473 (1/500) staining USP3 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview