All lanes : Anti-USP39 antibody (ab101902) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.