Anti-USP9x antibody
| Name | Anti-USP9x antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab19879 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB IHC-P ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human USP9x |
| Blocking Peptide | Human USP9x peptide |
| Description | Rabbit Polyclonal |
| Gene | USP9X |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immuofluorescent staining for USP9X in the rat hippocampus (dentate gyrus) using ab19879 (1/300 = 0.07µg/ml). Image is taken with X10 objective. ab19879 was incubated overnight at RT. Secondary antibody used was anti-rabbit Alexa fluor 488 (1/1000 for 2h at RT). Rats were intracardially perfused with paraformaldehyde 4%, brain tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cryostat for free floating IHC.
All lanes : Anti-USP9x antibody (ab19879) at 1 µg/mlLane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950)Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950) with Human USP9x peptide (ab20617) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab19879 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19879, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![USP9x was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to USP9x and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19879.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 290kDa: USP9x.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18882_USP9x-Primary-antibodies-ab19879-15.jpg)
USP9x was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to USP9x and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19879.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 290kDa: USP9x.
Product References
Ubiquitin-specific peptidase 9, X-linked (USP9X) modulates activity of mammalian - Ubiquitin-specific peptidase 9, X-linked (USP9X) modulates activity of mammalian
Agrawal P, Chen YT, Schilling B, Gibson BW, Hughes RE. J Biol Chem. 2012 Jun 15;287(25):21164-75.
ErbB2 trafficking and degradation associated with K48 and K63 polyubiquitination. - ErbB2 trafficking and degradation associated with K48 and K63 polyubiquitination.
Marx C, Held JM, Gibson BW, Benz CC. Cancer Res. 2010 May 1;70(9):3709-17.
Huntingtin interacting proteins are genetic modifiers of neurodegeneration. - Huntingtin interacting proteins are genetic modifiers of neurodegeneration.
Kaltenbach LS, Romero E, Becklin RR, Chettier R, Bell R, Phansalkar A, Strand A, Torcassi C, Savage J, Hurlburt A, Cha GH, Ukani L, Chepanoske CL, Zhen Y, Sahasrabudhe S, Olson J, Kurschner C, Ellerby LM, Peltier JM, Botas J, Hughes RE. PLoS Genet. 2007 May 11;3(5):e82.