USP9X

Gene Symbol USP9X
Entrez Gene 8239
Alt Symbol DFFRX, FAF, FAM, MRX99
Species Human
Gene Type protein-coding
Description ubiquitin specific peptidase 9, X-linked
Other Description Drosophila fat facets related, X-linked|deubiquitinating enzyme FAF-X|fat facets in mammals|fat facets protein related, X-linked|fat facets protein-related, X-linked|hFAM|probable ubiquitin carboxyl-terminal hydrolase FAF-X|ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila)|ubiquitin thioesterase FAF-X|ubiquitin thiolesterase FAF-X|ubiquitin-specific processing protease FAF-X|ubiquitin-specific protease 9, X chromosome|ubiquitin-specific-processing protease FAF-X
Swissprots Q8WWT3 Q8WX12 O75550 Q93008
Accessions EAW59406 EAW59407 EAW59408 EAW59409 EAW59410 EAW59411 EAW59412 Q93008 AA261797 AB209666 BAD92903 AF070645 AAC25395 AJ012078 AK294828 BAG57940 AW183065 BC014792 BC046205 AAH46205 BC054861 BC063645 AAH63645 BC172429 AAI72429 BE925861 BX956755 CD619095 DA854185 HG512500 X98296 CAA66942 XM_005272675 XP_005272732 XM_005272676 XP_005272733 NM_001039590 NP_001034679 NM_001039591 NP_001034680 NM_004652 NM_021906
Function Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Involved in axonal growth and neuronal cell migration. {ECO:0000269|PubMed:16322459, ECO:0000269|PubMed:18254724, ECO:0000269|PubMed:19135894, ECO:0000269|PubMed:24607389}.
Subcellular Location Cytoplasm. Cell projection, growth cone.
Tissue Specificity Widely expressed in embryonic and adult tissues.

USP9X (D4Y7W) Rabbit mAb - 14898 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4Y7W
Western blot analysis of extracts from various cell lines using USP9X (D4Y7W) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). LOX-IMVI cells are deficient in USP9X protein expression (13).

USP9X Antibody - 5751 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using USP9X Antibody.

USP9 (5G-02) - sc-100628 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
USP9 (5G-02): sc-100628. Western blot analysis of USP9 expression in HeLa nuclear extract.

USP9X/Y (G-20) - sc-55424 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

USP9X/Y (K-17) - sc-55425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

USP9X (P-20) - sc-55427 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
USP9X (P-20): sc-55427. Western blot analysis of USP9X (expression in HeLa (A), IMR-32 (B) and K-562 (C) whole cell lysates.

USP9X (M-20) - sc-55426 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

USP9X/Y (E-12) - sc-365353 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP9X/Y (E-12): sc-365353. Western blot analysis of USP9X/Y expression in Jurkat (A) and K-562 (B) whole cell lysates.

USP9X/Y (G-10) - sc-373818 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP9X/Y (G-10): sc-373818. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

USP9X/Y (H-300) - sc-134916 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-USP9x antibody - ab19879 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immuofluorescent staining for USP9X in the rat hippocampus (dentate gyrus) using ab19879 (1/300 = 0.07µg/ml). Image is taken with X10 objective. ab19879 was incubated overnight at RT. Secondary antibody used was anti-rabbit Alexa fluor 488 (1/1000 for 2h at RT). Rats were intracardially perfused with paraformaldehyde 4%, brain tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cryostat for free floating IHC.

Anti-USP9x antibody - ab26334 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab26334 stained HeLa cells. The cells were 100% Methanol fixed (5min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26334, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-USP9x antibody - ab56461 from Abcam

Host Mouse
Clonality Monoclonal

Anti-USP9x antibody - ab99343 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-USP9x antibody (ab99343) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH 3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-USP9x antibody - ab111046 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab111046, at 3.75 ug/ml, staining USP9x in formalin-fixed, paraffin-embedded Human brain cortex tissue. Stained using a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen

Anti-USP9x antibody [EPR13809(B)] - N-terminal - ab180191 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13809(B)
All lanes : Anti-USP9x antibody [EPR13809(B)] - N-terminal (ab180191) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-USP9X / FAM Antibody (clone 2G7) - LS-C174599 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G7
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP9X mouse monoclonal antibody.

Anti-USP9X / FAM Antibody (aa1150-1300, Biotin) - LS-C143431 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP9X / FAM Antibody (aa1150-1300, DY650) - LS-C143432 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP9X / FAM Antibody (aa1150-1300, HRP) - LS-C143434 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP9X / FAM Antibody (aa1150-1300, DY488) - LS-C143433 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP9X / FAM Antibody (aa1150-1300, DY550) - LS-C143435 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP9X / FAM Antibody - LS-C191377 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP9X / FAM Antibody (aa2497-2547) - LS-C288160 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-USP9X / FAM Antibody (aa625-675) - LS-C288161 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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