Anti-USP9x antibody
| Name | Anti-USP9x antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56461 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: MTATTRGSPV GGNDNQGQAP DGQSLPPLQQ NQTSSPDSSN ENSPATPPDE QGQGDAPPQL EDEEPAFPHT DLAKLDDMIN RPRWVVPVLP , corresponding to amino acids 1-91 of Human USP9x Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | USP9X |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab56461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18886_USP9x-Primary-antibodies-ab56461-1.jpg)
Overlay histogram showing HeLa cells stained with ab56461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
The SOX2-interactome in brain cancer cells identifies the requirement of MSI2 and - The SOX2-interactome in brain cancer cells identifies the requirement of MSI2 and
Cox JL, Wilder PJ, Gilmore JM, Wuebben EL, Washburn MP, Rizzino A. PLoS One. 2013 May 7;8(5):e62857.
Identifying specific protein interaction partners using quantitative mass - Identifying specific protein interaction partners using quantitative mass
Trinkle-Mulcahy L, Boulon S, Lam YW, Urcia R, Boisvert FM, Vandermoere F, Morrice NA, Swift S, Rothbauer U, Leonhardt H, Lamond A. J Cell Biol. 2008 Oct 20;183(2):223-39.