All lanes : Anti-USP9Y antibody [EPR13966] (ab181842) at 1/20000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : Molt-4 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling USP9Y with ab181842 at 1/200 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.