Anti-VASP (phospho S157) antibody [5C6]
| Name | Anti-VASP (phospho S157) antibody [5C6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab58555 |
| Prices | $381.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 5C6 |
| Applications | FC WB IP ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | A synthetic phosphopeptide corresponding to amino acids surrounding the Ser157 phosphorylation site of human VASP |
| Description | Mouse Monoclonal |
| Gene | VASP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19172_VASP-Primary-antibodies-ab58555-1.jpg)
Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
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Neukamm SS, Ott J, Dammeier S, Lehmann R, Haring HU, Schleicher E, Weigert C. J Biol Chem. 2013 Jun 7;288(23):16403-15.
Zyxin is a transforming growth factor-beta (TGF-beta)/Smad3 target gene that - Zyxin is a transforming growth factor-beta (TGF-beta)/Smad3 target gene that
Mise N, Savai R, Yu H, Schwarz J, Kaminski N, Eickelberg O. J Biol Chem. 2012 Sep 7;287(37):31393-405.