VASP

Gene Symbol VASP
Entrez Gene 7408
Alt Symbol -
Species Human
Gene Type protein-coding
Description vasodilator-stimulated phosphoprotein
Other Description -
Swissprots Q93035 P50552 B2RBT9 Q6PIZ1
Accessions CAA67109 CAA67147 EAW57362 EAW57363 EAW57364 EAW57365 P50552 AK314812 BAG37336 AL556269 BC026019 AAH26019 BC038224 AAH38224 BG751094 BG825983 Z46389 CAA86523 XM_005259199 XP_005259256 XM_005259200 XP_005259257 NM_003370 NP_003361 NM_001008736
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. {ECO:0000269|PubMed:10087267, ECO:0000269|PubMed:10438535, ECO:0000269|PubMed:15939738, ECO:0000269|PubMed:17082196, ECO:0000269|PubMed:18559661}.
Subcellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction {ECO:0000250}. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).
Tissue Specificity Highly expressed in platelets. {ECO:0000269|PubMed:7828592}.
Top Pathways Focal adhesion, Leukocyte transendothelial migration, Fc gamma R-mediated phagocytosis, cGMP-PKG signaling pathway, Rap1 signaling pathway

Phospho-VASP (Ser157) Antibody - 3111 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Forskolin #3828 (+), using Phospho-VASP (Ser157) Antibody (upper) and VASP (9A2) Rabbit mAb #3132 (lower).

Phospho-VASP (Ser239) Antibody - 3114 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 and C6 cells, untreated, 8-Br-cGMP-treated, 8-Br-cAMP-treated or forskolin-treated as indicated, using Phospho-VASP (Ser239) Antibody.

VASP (A290) Antibody - 3120 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types, using VASP (A290) Antibody.

VASP Antibody - 3112 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated THP-1 cells, untreated and forskolin treated C6 cells, and untreated C2C12 and COS cells, using the VASP Antibody.

VASP (9A2) Rabbit mAb - 3132 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 9A2
Western blot analysis of extracts from various cell lines using VASP (9A2) Rabbit mAb.

VASP (D-11) - sc-376226 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VASP (D-11): sc-376226. Western blot analysis of VASP expression in non-transfected: sc-117752 (A) and human VASP transfected: sc-114829 (B) 293T whole cell lysates.

VASP (C-17) - sc-1853 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VASP (C-17): sc-1853. Western blot analysis of VASP expression in MDCK (A) and A-10 (B) whole cell lysates.

VASP (H-90) - sc-13975 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VASP (H-90): sc-13975. Western blot analysis of VASP expression in HeLa (A) and COLO 320DM (B) whole cell lysates.

VASP (S-19) - sc-1950 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VASP (S-19): sc-1950. Western blot analysis of VASP expression in HeLa (A), A-431 (B), MDCK (C) and Mv 1 Lu (D) whole cell lysates.

VASP (A-11) - sc-46668 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VASP (A-11): sc-46668. Western blot analysis of VASP expression in ECV304 (A), A-431 (B) and HUV-EC-C (C) whole cell lysates.

p-VASP (A-7) - sc-365563 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of VASP phosphorylation in non-transfected: sc-117752 (A,D), untreated human VASP transfected: sc-114829 (B,E) and lambda protein phosphatase (sc-200312A) treated human VASP transfected: sc-114829 (C,F) 293T whole cell lysates. Antibodies tested include p-VASP (A-7): sc-365563 (A,B,C) and VASP (A-11): sc-46668 (D,E,F).

p-VASP (F-3) - sc-365564 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of VASP phosphorylation in non-transfected: sc-117752 (A,D), untreated human VASP transfected: sc-114829 (B,E) and lambda protein phosphatase (sc-200312A) treated human VASP transfected: sc-114829 (C,F) 293T whole cell lysates. Antibodies tested include p-VASP (F-3): sc-365564 (A,B,C) and VASP (A-11): sc-46668 (D,E,F).

p-VASP (Ser 239) - sc-23507 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-VASP (Ser 239): sc-23507. Western blot analysis of VASP phosphorylation expression in non-transfected: sc-117752 (A) and human VASP transfected: sc-114829 (B) 293T whole cell lysates.

p-VASP (Ser 157) - sc-101818 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-VASP (Ser 157): sc-101818. Western blot analysis of phosphorylated VASP expression in untreated (A) and forskolin-treated (B) NIH/3T3 whole cell lysates.

p-VASP (16C2) - sc-101439 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-VASP (16C2): sc-101439. Western blot analysis of VASP phosphorylation expression in non-transfected: sc-117752 (A) and human VASP transfected: sc-114829 (B) 293T whole cell lysates.

p-VASP (5C6) - sc-101440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-VASP (5C6): sc-101440. Western blot analysis of VASP phosphorylation expression in non-transfected: sc-117752 (A) and human VASP transfected: sc-114829 (B) 293T whole cell lysates.

Anti-VASP (phospho S156) antibody [EPR1065(2)] - ab109541 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1065(2)
All lanes : Anti-VASP (phospho S156) antibody [EPR1065(2)] (ab109541) at 1/1000 dilutionLane 1 : Human platelet cell lysate, untreatedLane 2 : Human platelet cell lysate treated with Alkaline Phosphatase (AP)Lysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Anti-VASP (phospho S157) antibody - ab47268 from Abcam

Prices $387.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab47268, at 1/50 dilution, staining VASP in Human tonsil tissue by Immunohistochemistry, Paraffin-embedded tissue. Left panel: no peptide; right panel: with immunizing phosphopeptide.

Anti-VASP (phospho S157) antibody - ab192211 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-VASP (phospho S157) antibody (ab192211) at 1/500 dilution (antigen-specific peptide)Lane 2 : Anti-VASP (phospho S157) antibody (ab192211) at 1/500 dilutionLane 1 : PMA treated HeLa whole cell lysates with Antigen-specific peptideLane 2 : PMA treated HeLa whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-VASP (phospho S157) antibody [5C6] - ab58555 from Abcam

Prices $381.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 5C6
Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-VASP (phospho S239) antibody - ab194747 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VASP (phospho S239) antibody (ab194747) at 1/500 dilutionLane 1 : Extract from serum-treated 293 cellsLane 2 : Extract from PMA-treated C6 cells

Anti-VASP antibody - ab47461 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VASP antibody (ab47461) at 1/1000 dilutionLane 1 : NIH/ 3T3 cells, treated with forskolin (40µM, 30min).Lane 2 : NIH/ 3T3 cells, treated with forskolin (40µM, 30min) pre-incubated with peptide.Lysates/proteins at 30 µg per lane.

Anti-VASP antibody - ab63528 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VASP antibody (ab63528) at 1/500 dilutionLane 1 : NIH3T3 cell extractsLane 2 : NIH3T3 cell extracts with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-VASP antibody - ab89829 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-VASP antibody (ab89829) at 1 µg/ml + human spleen tissue lysate at 50 µgdeveloped using the ECL technique

Anti-VASP antibody - ab47419 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VASP antibody (ab47419) at 1/500 dilutionLane 1 : Forskolin treated NIH/3T3 cell extracts with ForskolinLane 2 : Forskolin treated NIH/3T3 cell extracts with blocking peptideLysates/proteins at 30 µg per lane.
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