Anti-Vav proteins antibody [EP482Y]
| Name | Anti-Vav proteins antibody [EP482Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40875 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP482Y |
| Applications | WB FC ICC/IF ICC/IF IP |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to residues near the C-terminus of human Vav |
| Description | Rabbit Monoclonal |
| Gene | VAV3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate (10 ug).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19197_ab40875wb.jpg)
Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate (10 ug).
Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
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Gastaldello S, Chen X, Callegari S, Masucci MG. PLoS Pathog. 2013;9(10):e1003664.
Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation - Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation
Pauker MH, Hassan N, Noy E, Reicher B, Barda-Saad M. Sci Signal. 2012 Apr 24;5(221):rs3.
Casein kinase I delta controls centrosome positioning during T cell activation. - Casein kinase I delta controls centrosome positioning during T cell activation.
Zyss D, Ebrahimi H, Gergely F. J Cell Biol. 2011 Nov 28;195(5):781-97.
Cooperative interactions at the SLP-76 complex are critical for actin - Cooperative interactions at the SLP-76 complex are critical for actin
Barda-Saad M, Shirasu N, Pauker MH, Hassan N, Perl O, Balbo A, Yamaguchi H, Houtman JC, Appella E, Schuck P, Samelson LE. EMBO J. 2010 Jul 21;29(14):2315-28.
The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen - The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen
Gianni D, Bohl B, Courtneidge SA, Bokoch GM. Mol Biol Cell. 2008 Jul;19(7):2984-94.