Anti-VAV2 antibody [EP1067Y]

• Supplier: Abcam
• Catalog: ab52640
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1067Y
• Applications: WB IP ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on VAV2 (Human)
• Description: Rabbit Monoclonal
• Gene: VAV2
• Conjugate: Unconjugated

Product images

VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 100kDa; VAV2.

Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution + 293 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution

Product References

Paxillin kinase linker (PKL) regulates Vav2 signaling during cell spreading and - Paxillin kinase linker (PKL) regulates Vav2 signaling during cell spreading and

Jones MC, Machida K, Mayer BJ, Turner CE. Mol Biol Cell. 2013 Jun;24(12):1882-94.

Vav1-phospholipase C-gamma1 (Vav1-PLC-gamma1) pathway initiated by T cell antigen - Vav1-phospholipase C-gamma1 (Vav1-PLC-gamma1) pathway initiated by T cell antigen

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CIN85 is required for Cbl-mediated regulation of antigen receptor signaling in - CIN85 is required for Cbl-mediated regulation of antigen receptor signaling in

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The Aarskog-Scott syndrome protein Fgd1 regulates podosome formation and - The Aarskog-Scott syndrome protein Fgd1 regulates podosome formation and

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Synergistic signals for natural cytotoxicity are required to overcome inhibition - Synergistic signals for natural cytotoxicity are required to overcome inhibition

Kim HS, Das A, Gross CC, Bryceson YT, Long EO. Immunity. 2010 Feb 26;32(2):175-86.

Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. - Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.

Afshari FT, Kwok JC, Fawcett JW. J Neurosci. 2010 Mar 24;30(12):4246-55.

Eph receptor tyrosine kinases regulate astrocyte cytoskeletal rearrangement and - Eph receptor tyrosine kinases regulate astrocyte cytoskeletal rearrangement and

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Identification of the Eph receptor pathway as a novel target for eicosapentaenoic - Identification of the Eph receptor pathway as a novel target for eicosapentaenoic

Doleman JF, Eady JJ, Elliott RM, Foxall RJ, Seers J, Johnson IT, Lund EK. Nutr Metab (Lond). 2010 Jul 12;7:56.

The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen - The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen

Gianni D, Bohl B, Courtneidge SA, Bokoch GM. Mol Biol Cell. 2008 Jul;19(7):2984-94.