Anti-VCAM1 antibody [EPR5047]

• Supplier: Abcam
• Catalog: ab134047
• Prices: $99.00, $388.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5047
• Applications: WB IP IHC-P ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse VCAM1
• Blocking Peptide: VCAM1 peptide
• Description: Rabbit Monoclonal
• Gene: VCAM1
• Conjugate: Unconjugated

Product images

All lanes : Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified)Lane 1 : Mouse kidneyLane 2 : Rat spleenLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human tonsil with purified ab134047 at a dilution of 1/500. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunofluorescent staining of K562 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab134047 at a dilution of 1/250. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/2000 dilution (purified) + Rat kidney at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified) + NIH/3T3 at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified) + Human fetal liver at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/10000 dilution (purified) + HuT-78 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

IHC image of VCAM1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of VCAM1 staining in Human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab134047, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-VCAM1 antibody [EPR5047] (ab134047) at 1/1000 dilution (unpurified)Lane 1 : Human fetal liver lysateLane 2 : HuT 78 lysateLane 3 : NIH 3T3 lysateLane 4 : Mouse brain lysateLane 5 : Mouse kidney lysateLane 6 : Mouse spleen lysateLane 7 : Rat brain lysateLane 8 : Rat kidney lysateLane 9 : Rat spleen lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Product References

c-Myc is essential to prevent endothelial pro-inflammatory senescent phenotype. - c-Myc is essential to prevent endothelial pro-inflammatory senescent phenotype.

Florea V, Bhagavatula N, Simovic G, Macedo FY, Fock RA, Rodrigues CO. PLoS One. 2013 Sep 6;8(9):e73146.