Anti-VCP antibody
| Name | Anti-VCP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab111740 |
| Prices | $387.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human, Mouse, Rat, Chicken, Bovine, Pig, Xenopus, Zebrafish |
| Antigen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 290 and 507 of human VCP (P55072) |
| Description | Rabbit Polyclonal |
| Gene | VCP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-VCP antibody (ab111740) at 1/1000 dilutionLane 1 : NT2D1 whole cell lysateLane 2 : U87-MG whole cell lysateLane 3 : MCF7 whole cell lysateLysates/proteins at 30 µg per lane.
IHC image of VCP staining in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab111740, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunofluorescence analysis of VCP in paraformaldehyde fixed MCF7, using ab111740 at a 1/500 dilution. Lower image shows cells costained with Hoechst 33342.