VCP

Gene Symbol VCP
Entrez Gene 7415
Alt Symbol ALS14, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
Species Human
Gene Type protein-coding
Description valosin containing protein
Other Description 15S Mg(2+)-ATPase p97 subunit|TER ATPase|epididymis luminal protein 220|epididymis secretory protein Li 70|transitional endoplasmic reticulum ATPase|valosin-containing protein|yeast Cdc48p homolog
Swissprots B2R5T8 P55072 Q9UCD5 Q969G7 Q2TAI5 Q0V924
Accessions AAC07984 CAH56816 EAW58403 EAW58404 P55072 AF100752 AAD43016 AF313485 AAG29873 AF313486 AAG29874 AI343015 AK307735 AK312310 BAG35235 AL137377 CAB70717 BC007562 AAH07562 BC012195 BC017171 BC096751 BC110913 AAI10914 BC121794 AAI21795 BC122550 AAI22551 DA880782 DB524539 FJ224344 ACI46036 FJ224352 ACI46044 Z70768 CAA94809 NM_007126 NP_009057
Function Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmi
Subcellular Location Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Note=Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. Recruited to the cytoplasmic surface of the endoplasmic reticulum via interaction with AMFR/gp78. Following DNA double-strand breaks, recruited to the sites of damage. Recruited to stalled replication forks via interaction with SPRTN.
Top Pathways Protein processing in endoplasmic reticulum, Legionellosis

VCP (7F3) Rabbit mAb - 2649 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 7F3
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using VCP (7F3) Rabbit mAb.

VCP Antibody - 2648 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, K562, NIH/3T3, C6 and COS cells, using VCP Antibody.

VCP (G-8) - sc-374055 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VCP (G-8): sc-374055. Western blot analysis of VCP expression in non-transfected: sc-117752 (A) and human VCP transfected: sc-112114 (B) 293T whole cell lysates.

VCP (A-20) - sc-82766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VCP (H-120) - sc-20799 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VCP (H-120): sc-20799. Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.

VCP (N-12) - sc-21824 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VCP (N-12): sc-21824. Western blot analysis of VCP expression in A-431 (A), KNRK (B) and PC-12 (C) whole cell lysates.

VCP (G-20) - sc-9783 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VCP (G-20): sc-9783. Western blot analysis of VCP expression in NIH/3T3 whole cell lysate.

VCP (F-5) - sc-133211 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VCP (F-5): sc-133211. Western blot analysis of VCP expression in non-transfected 293T: sc-117752 (A), human VCP transfected 293T: sc-112114 (B), HeLa (C), NIH/3T3 (D) and PC-12 (E) whole cell lysates.

VCP (E-4) - sc-133125 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VCP (E-4): sc-133125. Western blot analysis of VCP expression in HeLa (A), MDA-MB-231 (B) and NIH/3T3 (C) whole cell lysates and rat brain tissue extract (D).

VCP (5) - sc-57492 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VCP (5): sc-57492. Western blot analysis of VCP expression in NIH/3T3 (A), MDA-MB-231 (B), HeLa (C), MCF7 (D), A-431 (E) and PC-12 (F) whole cell lysates.

VCP (D-9) - sc-133212 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VCP (D-9): sc-133212. Western blot analysis of VCP expression in non-transfected 293T: sc-117752 (A), human VCP transfected 293T: sc-112114 (B), HeLa (C), NIH/3T3 (D) and PC-12 (E) whole cell lysates.

VCP (18) - sc-136273 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
VCP (18): sc-136273. Immunofluorescence staining of HeLa cells showing cytoplasmic localization.

Anti-VCP antibody - ab111740 from Abcam

Prices $387.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VCP antibody (ab111740) at 1/1000 dilutionLane 1 : NT2D1 whole cell lysateLane 2 : U87-MG whole cell lysateLane 3 : MCF7 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-VCP antibody - ab155146 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VCP antibody (ab155146) at 1/2000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-VCP antibody - ab36047 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VCP antibody (ab36047) at 1 µg/mlLane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell LysateLane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-VCP antibody - ab76954 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HL60 cells stained with ab76954 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76954, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-VCP antibody - ab194965 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-VCP antibody (ab194965) at 0.01 µg/ml + HeLa cell lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-VCP antibody - ab84385 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84385 at 1/250 dilution staining VCP in human skin basal cell carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-VCP antibody - ab138298 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-VCP antibody (ab138298) at 1/500 dilution + HepG2 cell lysate at 30 µg

Anti-VCP antibody [3E8DC11] - ab110308 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E8DC11
Immunocytochemistry image of ab110308-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110308 at 2 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in nucleus and cytoplasm.

Anti-VCP antibody [4G9] - ab79037 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4G9
Immunohistochemistry analysis of formalin-fixed paraffin-embedded Human prostate tissue, using 5 µg/ml ab79037.

Anti-VCP antibody [5] - ab11433 from Abcam

Prices $404.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 5
Anti-VCP antibody [5] (ab11433) at 1/2000 dilution + whole cell lysate prepared from mouse embryonic fibroblasts at 20 µgSecondaryAlexa-Fluor 680 conjugated goat anti-mouse polyclonal at 1/10000 dilutionObserved band size : 97 kDa (why is the actual band size different from the predicted?)Image courtesy of an anonymous Abreview.See Abreview

Anti-VCP antibody [EPR3307(2)] - ab109240 from Abcam

Prices $99.00, $391.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3307(2)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab109240 at 1/100 dilution.

Anti-VCP antibody [EPR3308] - ab109198 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3308
All lanes : Anti-VCP antibody [EPR3308] (ab109198) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : A549 cell lysate Lane 3 : RAW264.7 cell lysateLysates/proteins at 10 µg per lane.

Anti-VCP Antibody (clone 1344CT150.163.114) - LS-C344135 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 1344CT150.163.114
Fluorescent image of U251 cells stained with VCP Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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