All lanes : Anti-VCP antibody (ab155146) at 1/2000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.
Anti-VCP antibody (ab155146) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Anti-VCP antibody (ab155146) at 1/10000 dilution + Rat brain lysate at 50 µg
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling VCP with ab155146 at 1/500 dilution.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling VCP with ab155146 at 1/500 dilution. Right panel co-stained with Hoechst 33342.