Anti-VCP antibody [5]
| Name | Anti-VCP antibody [5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11433 |
| Prices | $404.00 |
| Sizes | 50 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 5 |
| Applications | IHC-P IHC-F FC ELISA ICC/IF IP ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Sheep, Bovine, Human, Pig |
| Antigen | Synthetic peptide corresponding to Human VCP aa 792-806 |
| Description | Mouse Monoclonal |
| Gene | VCP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-VCP antibody [5] (ab11433) at 1/2000 dilution + whole cell lysate prepared from mouse embryonic fibroblasts at 20 µgSecondaryAlexa-Fluor 680 conjugated goat anti-mouse polyclonal at 1/10000 dilutionObserved band size : 97 kDa (why is the actual band size different from the predicted?)Image courtesy of an anonymous Abreview.See Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19309_VCP-Primary-antibodies-ab11433-20.jpg)
Anti-VCP antibody [5] (ab11433) at 1/2000 dilution + whole cell lysate prepared from mouse embryonic fibroblasts at 20 µgSecondaryAlexa-Fluor 680 conjugated goat anti-mouse polyclonal at 1/10000 dilutionObserved band size : 97 kDa (why is the actual band size different from the predicted?)Image courtesy of an anonymous Abreview.See Abreview
ab11433 staining VCP in Human H1299 cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 and were blocked in 10% serum at 25°C for 1 hour. Primary antibody was used at 1/1000 dilution (PBS) and incubated with sample for 1 hour at 37°C. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary at 1/1000 dilution. Image shows green color staining with ab11433 and nuclei were stained blue with DAPI.
ab11433 (1µg/ml) staining VCP in human left ventricle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear, cytoplasmic and cell membrane staining of cardiomyocutes and smooth muscle cells of cardiac artery.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab24762 detecting VCP in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The primary antibody was diluted 1/400 in 2% BSA in PBS and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-Mouse IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody. Abbreviations in the figure: P : Permeabilized; US Unstained, Red Peak; IgG Rb: IgG Mouse (Isotype Control), Blue Peak; VCP: Green Peak.See Abreview
Western blot of VCP from CA46 cell lysate using ab11433.
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human brain tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-VCP ab11433 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-VCP ab11433 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) ab11433 shows staining in C6 glioma cells. VCP staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP ab11433 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) ab11433 shows staining in WiDr colon carcinoma cells. VCP staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP ab11433 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunofluorescent analysis of VCP using VCP Monoclonal antibody (5) ab11433 shows staining in HeLa cells. VCP staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP ab11433 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Product References
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