Anti-VCP antibody [EPR3307(2)]
| Name | Anti-VCP antibody [EPR3307(2)] |
|---|---|
| Supplier | Abcam |
| Catalog | ab109240 |
| Prices | $99.00, $391.00 |
| Sizes | 10 µl, 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3307(2) |
| Applications | WB IP IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues in Human VCP |
| Description | Rabbit Monoclonal |
| Gene | VCP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab109240 at 1/100 dilution.
Immunofluorescent staining of HeLa cells using ab109240 at 1/100 dilution.
![All lanes : Anti-VCP antibody [EPR3307(2)] (ab109240) at 1/10000 dilutionLane 1 : MCF7 cell lysateLane 2 : HeLa cell lysateLane 3 : A549 cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg/ml per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19321_VCP-Primary-antibodies-ab109240-1.jpg)
All lanes : Anti-VCP antibody [EPR3307(2)] (ab109240) at 1/10000 dilutionLane 1 : MCF7 cell lysateLane 2 : HeLa cell lysateLane 3 : A549 cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg/ml per lane.
Overlay histogram showing HL60 cells stained with ab109240 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109240, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Effects of 1.8 GHz radiofrequency radiation on protein expression in human lens - Effects of 1.8 GHz radiofrequency radiation on protein expression in human lens
Zhang Y, Yao K, Yu Y, Ni S, Zhang L, Wang W, Lai K. Hum Exp Toxicol. 2013 Aug;32(8):797-806.
Valosin-containing protein (VCP/p97) is required for poliovirus replication and - Valosin-containing protein (VCP/p97) is required for poliovirus replication and
Arita M, Wakita T, Shimizu H. J Virol. 2012 May;86(10):5541-53.
Chemical chaperone TUDCA preserves cone photoreceptors in a mouse model of Leber - Chemical chaperone TUDCA preserves cone photoreceptors in a mouse model of Leber
Zhang T, Baehr W, Fu Y. Invest Ophthalmol Vis Sci. 2012 Jun 5;53(7):3349-56.