Flow Cytometry for ab179839 stained HeLa cells.Flow cytometry results of unstained cells (black), stained cells (0.2 µg/mL, red).
Immunocytochemistry with HDFn (100x) cells.HFDn cells treated with Antigen Retrieval (0.1 M Tris, 5% Urea pH 9.5 heated to 95⁰C incubated for 10 min) and stained with ab179839 (1.0 µg/mL, green); anti-HSP60 (1/3000, ab46798) and secondary antibody used was goat anti-rabbit Alexa® 594 (1/1000, ab150084, red); DAPI as nuclear stain (1/10000, blue).
Immunocytochemistry with HeLa cells (100x).HeLa cells treated with Antigen Retrieval (0.1 M Tris, 5% Urea pH 9.5 heated to 95⁰C incubated for 10 min) and stained with ab179839 (1.0 µg/mL, green); anti-HSP60 (1/3000, ab46798) and secondary antibody used was goat anti-rabbit Alexa® 594 (1/1000, ab150084, red); DAPI as nuclear stain (1/10000, blue).