Anti-VEGF 165B antibody
| Name | Anti-VEGF 165B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab90719 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human VEGF 165B |
| Description | Rabbit Polyclonal |
| Gene | VEGFA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-VEGF 165B antibody (ab90719) at 1 µg/ml + VEGF 165B Recombinant Protein at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab90719 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90719, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.