Anti-VEGF antibody

• Supplier: Abcam
• Catalog: ab46154
• Prices: $401.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-F IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human VEGF
• Description: Rabbit Polyclonal
• Gene: VEGFA
• Conjugate: Unconjugated

Product images

All lanes : Anti-VEGF antibody (ab46154) at 1 µgLane 1 : MEF whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : MCF7 whole cell lysateLane 4 : NIH 3T3 whole cell lysateLane 5 : RCC4 whole cell lysateLane 6 : PC12 whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-mouse IgG (IRDye 800) at 1/10000 dilutionPerformed under reducing conditions.

All lanes : Anti-VEGF antibody (ab46154) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Performed under reducing conditions.

ICC/IF image of this anti-VEGF antibody (ab46154) stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab46154, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ab46154 staining kidney tissue sections (human, mouse and rat) by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking with sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C.  The anti-VEGF antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit antibody was used as the secondary.See Abreview

ab46154 staining VEGF in HeLa cells treated with Y-27632 dihydrochloride (ab120129), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of Y-27632 dihydrochloride, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ab120129 (Y-27632 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab46154 staining VEGF in MCF7 cells treated with HA 1077 (ab120306), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of HA 1077 as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ab120306 (HA 1077) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

IHC image of VEGF staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46154, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ICC/IF image of ab46154 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46154, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Perturbed neural activity disrupts cerebral angiogenesis during a postnatal - Perturbed neural activity disrupts cerebral angiogenesis during a postnatal

Whiteus C, Freitas C, Grutzendler J. Nature. 2014 Jan 16;505(7483):407-11.

Effects of secreted factors in culture medium of annulus fibrosus cells on - Effects of secreted factors in culture medium of annulus fibrosus cells on

Moon HJ, Yurube T, Lozito TP, Pohl P, Hartman RA, Sowa GA, Kang JD, Vo NV. Osteoarthritis Cartilage. 2014 Feb;22(2):344-54.

CDK5 and its activator P35 in normal pituitary and in pituitary adenomas: - CDK5 and its activator P35 in normal pituitary and in pituitary adenomas:

Xie W, Wang H, He Y, Li D, Gong L, Zhang Y. Int J Biol Sci. 2014 Jan 25;10(2):192-9.

Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in - Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in

Moura CS, Lollo PC, Morato PN, Nisishima LH, Carneiro EM, Amaya-Farfan J. PLoS One. 2014 Jan 20;9(1):e83437.

The anti-tumor activity of a neutralizing nanobody targeting leptin receptor in a - The anti-tumor activity of a neutralizing nanobody targeting leptin receptor in a

McMurphy T, Xiao R, Magee D, Slater A, Zabeau L, Tavernier J, Cao L. PLoS One. 2014 Feb 28;9(2):e89895.

Murine experimental autoimmune encephalomyelitis is diminished by treatment with - Murine experimental autoimmune encephalomyelitis is diminished by treatment with

MacMillan CJ, Doucette CD, Warford J, Furlong SJ, Hoskin DW, Easton AS. PLoS One. 2014 Feb 26;9(2):e89770.

Epidermal or dermal specific knockout of PHD-2 enhances wound healing and - Epidermal or dermal specific knockout of PHD-2 enhances wound healing and

Zimmermann AS, Morrison SD, Hu MS, Li S, Nauta A, Sorkin M, Meyer NP, Walmsley GG, Maan ZN, Chan DA, Gurtner GC, Giaccia AJ, Longaker MT. PLoS One. 2014 Apr 2;9(4):e93373.

Mesenchymal stem cells from human fat engineered to secrete BMP4 are - Mesenchymal stem cells from human fat engineered to secrete BMP4 are

Li Q, Wijesekera O, Salas SJ, Wang JY, Zhu M, Aprhys C, Chaichana KL, Chesler DA, Zhang H, Smith CL, Guerrero-Cazares H, Levchenko A, Quinones-Hinojosa A. Clin Cancer Res. 2014 May 1;20(9):2375-87.

Local injection of deferoxamine improves neovascularization in ischemic diabetic - Local injection of deferoxamine improves neovascularization in ischemic diabetic

Wang C, Cai Y, Zhang Y, Xiong Z, Li G, Cui L. PLoS One. 2014 Jun 25;9(6):e100818.

miR-145 suppresses thyroid cancer growth and metastasis and targets AKT3. - miR-145 suppresses thyroid cancer growth and metastasis and targets AKT3.

Boufraqech M, Zhang L, Jain M, Patel D, Ellis R, Xiong Y, He M, Nilubol N, Merino MJ, Kebebew E. Endocr Relat Cancer. 2014 Aug;21(4):517-31.