![All lanes : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 3 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19624_VEGFC-Primary-antibodies-ab63221-1.jpg)
All lanes : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 3 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Lane 1 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 1 µg/mlLane 2 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/mlLane 1 : Human VEGFC full length protein (ab83573)Lane 2 : Human VEGFC full length protein (ab83573)Lysates/proteins at 0.1 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19625_VEGFC-Primary-antibodies-ab63221-2.jpg)
Lane 1 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 1 µg/mlLane 2 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/mlLane 1 : Human VEGFC full length protein (ab83573)Lane 2 : Human VEGFC full length protein (ab83573)Lysates/proteins at 0.1 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HepG2 cells stained with ab63221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63221, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19626_VEGFC-Primary-antibodies-ab63221-5.jpg)
Overlay histogram showing HepG2 cells stained with ab63221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63221, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.