ab101760 staining VGLUT2 in Xenopus laevis retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in TBS with 5% BSA and 0.1% Triton-X 100) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-goat IgG polyclonal (1/1000) was used as the secondary antibody. Top - VGLUT2 (green), Middle - VGLUT1 (red), Bottom - merge.See Abreview
ab101760 staining VGLUT2 in Mouse thalamocortical terminals tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. The sample was incubated with primary antibody (1/500 in TBS with 5% BSA and 0.1% Triton-X 100) at 4°C for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-goat polyclonal (1/500) was used as the secondary antibody. Left - VGLUT2 (green), Middle - mGluR2 (red), Right - merge.See Abreview
ab101760 staining VGLUT2 in Rat dopaminergic (DA) neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% serum for 20 minutes at 23°C. Samples were incubated with primary antibody (1/500 in TBS with 5% BSA + 0.1% Triton-X 100) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-goat IgG polyclonal (1/1000) was used as the secondary antibody. Left - VGLUT2 (green), Middle - Tyrosine hydroxylase (red), Left - Merged.See Abreview
IHC - Wholemount of Rat lumbar dorsal root ganglion labelling VGLUT2 with ab101760. Sample was incubated with primary antibody (1/200 in TBS with 5% BSA + 0.1% Triton-X 100) for 12 hours at 4°C. An Alexa Flour® 488-conjugated Donkey anti-goat IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview