Anti-Villin antibody [CWWB1]
| Name | Anti-Villin antibody [CWWB1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3304 |
| Prices | $384.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | CWWB1 |
| Applications | ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Mouse, Human |
| Antigen | Full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | VIL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing Caco 2 cells stained with ab3304 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3304, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Caco 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19817_Villin-Primary-antibodies-ab3304-1.jpg)
Overlay histogram showing Caco 2 cells stained with ab3304 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3304, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Caco 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Overactivation of intestinal SREBP2 in mice increases serum cholesterol. - Overactivation of intestinal SREBP2 in mice increases serum cholesterol.
Ma K, Malhotra P, Soni V, Hedroug O, Annaba F, Dudeja A, Shen L, Turner JR, Khramtsova EA, Saksena S, Dudeja PK, Gill RK, Alrefai WA. PLoS One. 2014 Jan 20;9(1):e84221.
Serotonin receptor diversity in the human colon: Expression of serotonin type 3 - Serotonin receptor diversity in the human colon: Expression of serotonin type 3
Kapeller J, Moller D, Lasitschka F, Autschbach F, Hovius R, Rappold G, Bruss M, Gershon MD, Niesler B. J Comp Neurol. 2011 Feb 15;519(3):420-32.
Energy-dependent regulation of cell structure by AMP-activated protein kinase. - Energy-dependent regulation of cell structure by AMP-activated protein kinase.
Lee JH, Koh H, Kim M, Kim Y, Lee SY, Karess RE, Lee SH, Shong M, Kim JM, Kim J, Chung J. Nature. 2007 Jun 21;447(7147):1017-20. Epub 2007 May 7.