VIL1

Gene Symbol VIL1
Entrez Gene 7429
Alt Symbol D2S1471, VIL
Species Human
Gene Type protein-coding
Description villin 1
Other Description villin-1
Swissprots Q96AC8 B2R9A7 Q53S11 P09327
Accessions AAY14886 CAA00664 CAA50375 CAJ46927 EAW70619 EAW70620 P09327 A14103 CAA01148 AK223398 BAD97118 AK300965 BAG62590 AK313709 BAG36454 BC017303 AAH17303 BP329402 X04657 CAA28355 X12901 CAA31386 NM_007127 NP_009058
Function Epithelial cell-specific Ca(2+)-regulated actin- modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity
Subcellular Location Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip {ECO:0000250}. Cell projection, filopodium {ECO:0000250}. Note=Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. {ECO:0000250}.
Tissue Specificity Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level). {ECO:0000269|PubMed:14550699, ECO:0000269|PubMed:3453110}.

Villin-1 (R814) Antibody - 2369 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT29 cells and mouse kidney using Villin-1 (R814) Antibody.

Villin-1 Antibody - 2393 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT29 and HCT15 cells, using Villin-1 Antibody.

Villin (B-12) - sc-373997 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Villin (B-12): sc-373997. Western blot analysis of Villin expression in HCT 8 whole cell lysate.

Villin (BDID2C3) - sc-66022 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Villin (BDID2C3): sc-66022. Western blot analysis of Villin expression in human colon (A) and mouse kidney (B) tissue extracts.

Villin (E-12) - sc-374350 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Villin (E-12): sc-374350. Western blot analysis of Villin expression in non-transfected: sc-117752 (A) and mouse Villin transfected: sc-124565 (B) 293T whole cell lysates.

Villin (G-6) - sc-365310 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Villin (G-6): sc-365310. Western blot analysis of Villin expression in HCT-8 whole cell lysate.

Villin (H-60) - sc-28283 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Villin (H-60): sc-28283. Western blot analysis of Villin expression in mouse kidney tissue extract.

Villin (C-19) - sc-7672 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Villin (C-19): sc-7672. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing localization within the cytoplasm (A).Immunofluorescence staining of methanol-fixed PC-12 cells showing cytoskeletal localization (B).

Villin (V-20) - sc-33347 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Villin (V-20): sc-33347. Western blot analysis of Villin expression in non-transfected: sc-117752 (A) and mouse Villin transfected: sc-124565 (B) 293T whole cell lysates.

Villin (1D2C3) - sc-58897 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Villin (1D2C3): sc-58897. Western blot analysis of Villin expression in human colon tumor tissue (A,C) and mouse kidney tissue (B,D) under reducing (A,B) and non-reducing (C,D) conditions.

Villin (12) - sc-136119 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Villin (12): sc-136119. Western blot analysis of Villin expression in HCT-8 whole cell lysate.

Villin (CWWB1) - sc-58898 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
Villin (CWWB1): sc-58898. Western blot analysis of Villin expression in human colon tumor tissue extract.

Anti-Villin antibody - ab203296 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of formalin-fixed and paraffin embedded rat kidney labeling Villin with ab203296 at 1/200 dilution with DAB staining.

Anti-Villin antibody - ab52102 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab52102 at 1/50 dilution staining human Villin in small intestine by Immunohistochemistry, Formalin-fixed Paraffin embedded tissue.

Anti-Villin antibody [5E3B2] - ab202021 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5E3B2
Anti-Villin antibody [5E3B2] (ab202021) at 1/500 dilution + Recombinant fragment of Villin (aa 1-209) at 1 µg

Anti-Villin antibody [CWWB1] - ab3304 from Abcam

Prices $384.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone CWWB1
Overlay histogram showing Caco 2 cells stained with ab3304 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3304, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Caco 2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Villin antibody [EPR3490] - ab109516 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3490
All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilutionLane 1 : Human small intestine lysateLane 2 : HT-29 lysateLane 3 : Caco-2 lysateLysates/proteins at 10 µg per lane.

Anti-Villin antibody [EPR3491(3)] - ab133510 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3491(3)
All lanes : Anti-Villin antibody [EPR3491(3)] (ab133510) at 1/10000 dilutionLane 1 : CaCo-2 lysateLane 2 : HT-29 lysateLane 3 : Human small intestine lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilution

Anti-Villin antibody [M245] - ab86704 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone M245
Anti-Villin antibody [M245] (ab86704) at 1/250 dilution + Adult mouse kidney lysate

Anti-Villin antibody, prediluted - ab52135 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab52135, used neat, staining Human Villin in Small intestine using Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

Anti-Villin antibody [SPM226], prediluted - ab54194 from Abcam

Prices $376.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone SPM226
Staining of Villin in Human kidney tissue by immunohistochemistry using ab54194.

Anti-Villin antibody - N-terminal - ab135608 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Villin antibody - N-terminal (ab135608) at 1/50 dilution + Mouse kidney tissue lysates at 35 µg

Anti-VIL1 / Villin Antibody (C-Terminus) - LS-C76819 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VIL1 / Villin Antibody (clone SPM178) - LS-C189286 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone SPM178

Anti-VIL1 / Villin Antibody (clone CWWB1) - LS-C202916 from LifeSpan Bioscience

Prices $475.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal
Clone CWWB1
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