Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labelling Vimentin, using ab137321> at a 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded, formalin-fixed RT2 rat xenograft tissue labelling Vimentin, using ab137321> at a 1/500 dilution.
Confocal immunofluorescence analysis of methanol-fixed HeLa cells labelling Vimentin, using ab137321> (green) at a 1/500 dilution. Alpha-tubulin filaments were labelled with an anti-alpha tubilin antibody (red) at a 1/2000 dilution.
Anti-Vimentin antibody (ab137321) at 1/3000 dilution + 293T whole cell lysate at 30 µg
Anti-Vimentin antibody (ab137321) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
All lanes : Anti-Vimentin antibody (ab137321) at 1/3000 dilutionLane 1 : 293T whole cell lysate at 20 µgLane 2 : 293T whole cell lysate at 10 µgLane 3 : 293T whole cell lysate at 5 µg