VIM

Gene Symbol VIM
Entrez Gene 420519
Alt Symbol -
Species Chicken
Gene Type protein-coding
Description vimentin
Other Description -
Swissprots P09654 Q91023
Accessions AAA49134 AAW49254 AAW49255 P09654 NM_001048076 NP_001041541
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Anti-Vimentin antibody - ab137321 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labelling Vimentin, using ab137321> at a 1/500 dilution.

Anti-Vimentin antibody - ab192691 from Abcam

Prices $370.00
Sizes 50 µl
Clonality Polyclonal

Anti-Vimentin antibody [MM0952-11E18] - ab201637 from Abcam

Prices $359.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone MM0952-11E18

Anti-Vimentin antibody [RV202] - ab8978 from Abcam

Prices $404.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone RV202
ab8978 vimentin staining of a tonsilar lymphoma. Note that the epithelium (at the left) is negative.

Anti-Vimentin antibody [RV202] (FITC) - ab128507 from Abcam

Prices $385.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone RV202
ab128507, at 1/200 dilution, staining Vimentin in a frozen section of Swine colon tissue by Immunohistochemistry.

Anti-Vimentin antibody [V9] - ab80667 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone V9
ab80667 at 1/100 dilution staining Vimentin in osteosarcoma by Immunohistochemistry, Formalin fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Anti-Vimentin antibody [V9] (Biotin) - ab79032 from Abcam

Prices $376.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone V9
ab79032 at 1/100 dilution staining Vimentin in human osteosarcoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells. The purified form of this antibody was used for staining.

Anti-Vimentin antibody [V9], prediluted - ab74182 from Abcam

Prices $378.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone V9
Formalin-fixed, paraffin-embedded human osteosarcoma stained with ab74182 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Anti-Vimentin antibody [V9] - Cytoskeleton Marker - ab8069 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone V9
ab8069 staining Vimentin in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8069 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Vimentin antibody [V9] - Cytoskeleton Marker (Alexa Fluor® 488) - ab195877 from Abcam

Prices $431.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone V9
ab195877 staining Vimentin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195877 at 1/1000 dilution (shown in green) and ab6046, Rabbit polyclonal to beta Tubulin at 1µg/ml overnight at +4°C. Ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed, was then incubated at 2µg/ml for 1h at room temperature (shown in red)  Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Vimentin antibody [V9] - Cytoskeleton Marker (Alexa Fluor® 647) - ab195878 from Abcam

Prices $431.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone V9
ab195878 staining Vimentin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195878 at 1/5000 dilution(shown in red) and ab6046, Rabbit polyclonal to beta Tubulin at 1µg/ml overnight at +4°C. ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, was then incubated at 2µg/ml for 1h at room temperature (shown in green) Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Vimentin antibody [VI-01] - ab7752 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone VI-01
Ab7752 staining human kidney tissue. Staining can be observed in the cytoplasm of cells.Left panel: with primary antibody at 2ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Vimentin antibody [VI-10] - ab20346 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone VI-10
Human normal skin. Staining is localised to cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Vimentin antibody [VI-10] - ab78111 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone VI-10
Overlay histogram showing HeLa cells stained with ab78111 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78111, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

Anti-Vimentin antibody [Vim 3B4] - ab28028 from Abcam

Prices $392.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone Vim 3B4
ab28028 staining of tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-Vimentin antibody [VM452] - ab187380 from Abcam

Prices $379.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone VM452
Immunohistochemistry anlaysis of formalin/PFA-fixed paraffin-embedded Human melanoma tissue labeling Vimentin with ab187380 at 1µg/ml.

Anti-Vimentin antibody - C-terminal - ab194131 from Abcam

Prices $370.00
Sizes 50 µl
Clonality Polyclonal

Anti-Vimentin antibody - Cytoskeleton Marker - ab45939 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Vimentin antibody - Cytoskeleton Marker (ab45939) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Vimentin antibody - 10R-2386 from Fitzgerald

Prices $180.00
Sizes 5 ml
Host Mouse
Clonality Monoclonal
Clone VIM 3B4

Vimentin Monoclonal Antibody (RV202) - OMA1-06001 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone RV202
Immunofluorescent analysis of 1 month old zebrafish embryo using Vimentin monoclonal antibody (Product # OMA1-06001).

Vimentin Monoclonal Antibody (VI-10) - MA1-10459 from Thermo Fisher Scientific

Prices $225.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone VI-10
Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).

Anti-Vimentin Antibody, clone 3CB2 - MABT121 from EMD Millipore

Prices $305.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3CB2

AMF-17b - AMF-17b from Developmental Studies Hybridoma Bank

Host Mouse
Clonality Monoclonal
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