Anti-Vimentin antibody [VI-01]
| Name | Anti-Vimentin antibody [VI-01] |
|---|---|
| Supplier | Abcam |
| Catalog | ab7752 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | VI-01 |
| Applications | ELISA ELISA ICC/IF WB ICC/IF ICC/IF IHC-P IP FC |
| Species Reactivities | Mouse, Rat, Chicken, Hamster, Bovine, Human, Pig, Broad species, Mammalian |
| Antigen | Pellet of pig brain cold stable proteins after depolymerization of microtubules |
| Description | Mouse Monoclonal |
| Gene | VIM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab7752 staining human kidney tissue. Staining can be observed in the cytoplasm of cells.Left panel: with primary antibody at 2ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Image from Ermakova S et al, J Biol Chem 280:16882-90 (2005), Fig 4.
ICC/IF image of ab7752 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7752, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab7752 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100® for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7752, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100® used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19969_Vimentin-Primary-antibodies-ab7752-5.jpg)
Overlay histogram showing HeLa cells stained with ab7752 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100® for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7752, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100® used under the same conditions.
Product References
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