Anti-Vimentin antibody [VI-10]
| Name | Anti-Vimentin antibody [VI-10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab20346 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | VI-10 |
| Applications | ICC/IF ICC/IF IP WB ICC/IF FC |
| Species Reactivities | Mouse, Rat, Chicken, Dog, Human, Pig |
| Antigen | Full length native protein (purified) |
| Description | Mouse Monoclonal |
| Gene | VIM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human normal skin. Staining is localised to cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunofluorescence staining of RBL rat basophiilioc cell line with anti-Vimentin (VI--10). Nuclei are stained with DAPI (blue).
ab20346 at a 1/1000 dilution staining rat astrocyte cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-mouse Alexa-Fluor ® 568 (ab175473).This image is courtesy of an Abreview submitted by Randal Moldrich on 8 February 2006See Abreview
ab20346 at a 1/500 dilution staining mouse dissociated neural precursor cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-mouse Alexa-Fluor 568 (red stain) ab175473>ab175473). The image also shows a blue nuclear counterstain. Confocal image is shown with 2x zoom detail in top right corner.This image is courtesy of an Abreview submitted by Randal Moldich on 22 February 2006.See Abreview
![Overlay histogram showing NIH3T3 cells stained with ab20346 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20346, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NIH3T3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19975_Vimentin-Primary-antibodies-ab20346-4.jpg)
Overlay histogram showing NIH3T3 cells stained with ab20346 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20346, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NIH3T3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
Product References
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Overexpression of the skNAC gene in human rhabdomyosarcoma cells enhances their - Overexpression of the skNAC gene in human rhabdomyosarcoma cells enhances their
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Variable metastatic potentials correlate with differential plectin and vimentin - Variable metastatic potentials correlate with differential plectin and vimentin
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Alternative mRNA splicing from the glial fibrillary acidic protein (GFAP) gene - Alternative mRNA splicing from the glial fibrillary acidic protein (GFAP) gene
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Reactive astrocytes overexpress TSPO and are detected by TSPO positron emission - Reactive astrocytes overexpress TSPO and are detected by TSPO positron emission
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