ab115496, at 15 µg/ml, staining Von Hippel Lindau in formalin-fixed, paraffin-embedded Human Pancreas tissue by Immunohistochemistry using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
![Anti-Von Hippel Lindau antibody [52A11] (ab115496) at 1/500 dilution + HepG2 cell lysateSecondaryGoat anti-mouse secondary antibody conjugated to HRP developed using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/20262_Von-Hippel-Lindau-Primary-antibodies-ab115496-2.jpg)
Anti-Von Hippel Lindau antibody [52A11] (ab115496) at 1/500 dilution + HepG2 cell lysateSecondaryGoat anti-mouse secondary antibody conjugated to HRP developed using the ECL technique
![Overlay histogram showing HepG2 cells stained with ab115496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115496, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/20263_Von-Hippel-Lindau-Primary-antibodies-ab115496-4.jpg)
Overlay histogram showing HepG2 cells stained with ab115496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115496, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.